HPLC测定肝达片中熊果酸的含量

Determination of ursolic acid in Ganda tablet by HPLC

目的建立测定肝达片中熊果酸含量的HPLC方法.方法采用ODS柱,流动相为甲醇-水-磷酸(86∶14∶0.1),流速1.0 ml·min-1,检测波长208 nm,进样量20 μl.结果熊果酸在0.4～4.0 μg范围内呈良好的线性关系(r=0.9996),平均回收率为101.7%,RSD=1.24%.结论所用方法准确、可靠、简便,精密度好,可对肝达片进行质量控制.

OBJECTIVE To develop an HPLC method for determination of ursolie acid in Ganda tablet. METHODS ODS column was used, mobile phase consisted of methanol - water- phosphoric acid （86：14：0.1 ）, the flow rate was 1.0ml·min^-1 and detection wavelength was set at 208 nm. RESULTS Ursolic acid presented a good linear correlation within the range of 0.4μg to 4.0μg（ r=0. 9996）. The average recovery was 101.7%,with RSD of 1.24%.CONCLUSION The method is accurate, reliable, simple and precise, whieh can be used for quality control of Ganda tablet.