杭白芍过氧化物酶同工酶及芍药苷HPLC图谱分析

Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin i n Hangzhou white peony

采用聚丙烯酰胺凝胶电泳法分离杭白芍的过氧化物酶(POD)同工酶,经活性染色后显示:杭白芍13个品系的POD同工酶谱带数为2～5条,相对迁移率分布在0.567～0.685之间,根据酶谱带数和相对迁移率可归为3个类型.高压液相色谱(HPLC)法测定结果表明:杭白芍以芍药苷为主,仅含少量的芍药内酯苷,并且未出现氧化芍药苷的吸收峰;不同炮制方法加工的杭白芍样品的芍药苷含量存在差异,但芍药苷HPLC图谱基本相同.

The peroxidase isozymes of Hangzhou white peony were isolated by polyacrylamide gel electrophoresis. Up to 2-5 active bands of peroxidase isozyme were observed in 13 lines after active dyeing, and the rate of relative migration was located in the range from 0. 567 to 0. 685. Based on the differences in number and location of bands the individuals were divided into 3 groups. The results of HPLC showed that paeoniflorin was the primary component in Hangzhou white peony, and only a little albiflorin with no oxypaeoniflorin was observed. The paeoniflorin content of Hangzhou white peony as detected after different processing was different, although the HPLC chromatograms of paeoniflorin were similar.