表达黄瓜花叶病毒外壳蛋白的转基因番茄及其对CMV的抗性

Transgenic Tomato Plants Expressing Cucumber Mosaic Virus Coat Protein and Their Resistance to

利用根瘤土壤杆菌Ｔｉ质粒转化系统，采用叶盘法将ＣＭＶ－Ｃｐ基因转入番茄细胞中，获得４２株转基因植株，经Ｓｏｕｔｈｅｒｎｂｌｏｔ和Ｗｅｓｔｅｒｎｂｌｏｔ检测，证明ＣＭＶ－ｃｐ基因已进入番茄核染色体中，并能正常表达。通过对转基因植株Ｒ１和Ｒ２代苗期人工接种ＣＭＶ鉴定，发现ＣＭＶ－Ｃｐ基因产物对ＣＭＶ有一定抗性，其抗性遗传符合孟德尔一对基因控制性状的遗传规律并能稳定遗传。

A chimeric gene containing a cloned cDNA of the coat protein(cp)of cucumber mosaic virus(CMV)was transferred Into tomato cells,and 42 transgenic tomato plants have been produced by means of a novel leaf disk transformation with the help of a modified Ti plasmid of Agrobacterium tumefaciens.The results of DNA Southern blot analysis confirmed that the CMV-cp gene was stably integrated into the genome of the transgenic plants.CMV coat protein was expressed in the plant cells tested by Western blot and Dot blot.It was shown that the transgenic plants of tomato were protected from infection by CMV.The rate of systematic infection of the virus Is reduced or delayed compared With the control plants.The segregation of the R1 progeny of CMV resistance in a 3:1 ratio indicates a single insertion inherited in a Mendelian fashion.