冠心病外周血单核细胞肺炎衣原体病原体检测的意义

Detection of Pneumonia Chlamydiae in the Peripheral Blood Mononuclear Cell of Coronary Heart Disease by Indirect Immunofluorescence and Nest-PCR

目的:探讨冠心病患者外周血单核细胞(PBMC)中肺炎衣原体(Cpn)病原体检测的方法及临床意义.方法:选择150例冠心病(冠心病组)及55例非冠心病(对照组)患者,使用低渗溶血法制备PBMC,直接免疫荧光(DIF)检测Cpn包涵体,巢式PCR(nPCR)法测定外周血单核细胞中肺炎衣原体DNA.结果:150例冠心病患者Cpn包涵体阳性20例,阳性率13.3%,55例对照组中仅1例阳性,阳性率为1.8%,Cpn DNA两组间阳性率分别32.7%(49/150)及1.8%(1/55),冠心病组与对照组比较包涵体及DNA检测的阳性率明显增高,(相对危险度分别为26.2、8.3,95%的可信区间分别为3.52～194.98,1.09～63.46).结论:Cpn病原体阳性反映患者体内存在Cpn感染状态,包涵体与DNA检测两者间具有一致的阳性检出,但包涵体检测更省时省力.

Objective： To detect pneumoniae chlamydia in peripheral blood mononudear cell （PBMC）.Methods： The PBMC were isolate form 3 ml of the whole blood specimen by low permeate hemolysis method, The inclusion bodies was determined by direct immunofluorescence（DIF） and the DNA by nest - PCR in 150 cases with coronary heart disease（CHD） and 55 non CHD（control group, CG）. Results： Pneumoniae chlamydia Inolusion bodies was detected in 20（ 13.3% ） of 150 patients with coronary heart disease and in 1 （1.8 % ） of 55 in control group. Cpn DNA positive rats was 32.7 % （49/150） in CHD and 1.8 % （1/55） in control group .The positivity rates of chlamydia pneumoniae inclusion bodies and the Cpn DNA in CHD groups were significantly higher than those in control group. Conclusion： Pncumomae chlamydia inclusion bodies and DNA were present in PBMC among the significant proportion of CHD patients. PBMC sample can be collected easily and trustworthily. The positive result in PBMC is showing evidence for the presence of Cpn and is infecting.