PD-ECGFmRNA在食管癌中表达的临床意义

The Clinical Significance of the Expression of PD-ECGFmRNA in Esophageal Carcinoma

目的:探讨血小板衍化内皮细胞生长因子(PD-ECGF)的mRNA表达与食管癌侵袭性的关系.方法:用逆转录聚合酶链反应(RT-PCR)法测定40例食管鳞癌、23例食管腺癌和13例正常食管组织中的PD-ECGF mRNA的水平,分析其与癌细胞类型、分化程度及术后临床病理分期(PTNM)的关系.结果:食管癌组织中PD-ECGF明显较正常食管组织升高(t=3.21,P<0.05),食管腺癌中的PD-ECGFmRNA表达水平是食管鳞癌的2.36倍(t=2.12,P<0.05),所有3级分化癌组织中PD-ECG FmRNA的表达水平是1～2级的2.56倍(t=3.18,P<0.05),而与术后临床病理分期(TNM)无明显相关.结论:PD-ECG FmRNA在食管癌中的表达水平与其细胞类型及分化程度相关,临床治疗应采取相应的方案.

Objective： To examine the expression of platelet- derived endothelial cell growth factor （PD- ECGF） mRNA in esophageal carcinoma arid analyse the relationship between the exprssion of PD - ECGF and the invasion of the carcinoma. Methods：The level of mRNA expression was examined by reverse transcription- polymerase chain reaction （RT- PCR）. Results： There was significant difference between nomrality and esophageal careinonra tissue in expression of PD - ECG FmRNA. The mRNA level of PD- ECGF in adenocarcinomas was 2.36 times as high as that of squamas carcinomas（ t = 2.12, P 〈 0.05）. The level of PD -ECGF in grade 3 carcinoma was 2.56 times as high as that of Grade 1 ～ 2（ t = 3.18, P 〈 0.05） .There was no correlation between the level of PD - ECGF and PTNM. Conclusion： There is positively conelation among the level of PD - ECGF and the kind of cell of tumors and the grade of carcinoma. The clinical therapy should be made respectively.