白细胞介素-6基因型及血清水平与急性心肌梗死的关系

Interleukin-6 serum level and genotypes in patients with acute myocardial infarction

目的分析急性心肌梗死患者白细胞介素-6(IL-6)基因启动子-572C/G、-634C/G的多态性,探讨IL-6的基因型及其血清水平与急性心肌梗死的关系.方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的分析方法,检测了128例急性心肌梗死患者和145例健康对照者IL-6基因多态性,同时采用酶联免疫吸附试验检测IL-6的血清水平.结果急性心肌梗死组IL-6水平显著高于对照组(P＜0.01),IL-6-572C/G基因型和等位基因频率在急性心肌梗死组和对照组比较,差异有显著性(P＜0.05),与健康对照组相比,发现G等位基因携带者患急性心肌梗死的的相对风险度增加1.740倍(OR=1.740,95%CI=1.135～2.668).携带G等位基因的急性心肌梗死患者血清IL-6水平显著高于不携带者(P＜0.01).IL-6基因-634C/G多态性在急性心肌梗死组和对照组之间比较差异无显著性(P＞0.05).结论IL-6基因-572C/G多态性与急性心肌梗死之间存在相关关系,其中G等位基因可能是急性心肌梗死发病的遗传易感基因;携带G等位基因的个体可能通过促进IL-6的高度表达进而增加了急性心肌梗死的发病风险.

Objective To analyze polymorphism of the interleukin-6 （IL-6） gene promoter-572C/G and -634C/G in patients with acute myocardial infarction （AMI）, and to study the relation between serum levels and genotype of interleukin-6. Methods The polymorphism of IL-6 was detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method in 128 AMI patients and 145 healthy persons, The serum level of IL-6 was determined by enzyme-linked immanosorbent assay （ELISA）. Results. AMI group showed significantly higher serum level of IL-6 than control group （ P 〈 0.01 ）. There was a significant difference in frequencies of allele and genotype in IL-6 gene-572C/G polymorphism between AMI and control groups （ P 〈 0.01 ）. IL-6 allele G carriers increased onset risk with 1.740 fold for AM1 （OR = 1.740, 95% CI = 1. 135-2.668） compared with healthy persons. The distribution of IL-6 gene-634C/G polymorphism was not different between AMI group and control group （P 〉 0.05 ）. Conclusion IL-6 gene-572C/G polymorphism is associated with AMI. The IL-6 allele G carriers may be at increased risk of AMI due to enhanced the IL-6 expression.