幽门螺杆菌与胆囊结石形成关系的研究

Correlation between Helicobacter pylori and human gallstones

目的研究胆囊幽门螺杆菌感染与胆囊结石形成的关系。方法采用对照研究方法,对35例单纯胆囊结石(实验组)和25例单纯胆囊息肉样病变(对照组)的胃幽门螺杆菌(Helicobacterpylori,Hp)进行检测,用PCR方法检测2组患者的胆汁及结石Hp细胞毒素相关基因抗原(cagA)。结果2组患者胃Hp感染率分别为51·4%(18/35)、48·0%(12/25),差异无显著性(χ2=0·069,P=0·793)。实验组胆汁标本中cagA7例阳性(20·0%,7/35),结石标本中1例阳性(2·9%,1/35),对照组胆汁标本无一例阳性,2组胆汁cagA检出率差异有显著性(χ2=5·822,P=0·016),胆汁中cagA检出情况与胃幽门螺杆菌感染有关(χ2=3·886,P=0·049)。结论幽门螺杆菌DNA存在于胆囊结石患者的胆汁及结石中,与胃Hp感染有关,胆囊Hp感染与胆囊结石形成有关。

Objective To study the relationship between the Helicobacter pylori （Hp） injection and the tormahon of gallstones. Methods In this controlled study, the Hp infection rate was detected in both 35 cases of simple gallbladder stones （Experimental Group） and 25 cases of polypoid lesions of the gallbladder （Control Group）. The Hp cytotoxin associated gene antigen （cag-A） in bile and gallstones of the both groups was amplified by PCR technique. Results The Hp infection rate was 51.4%（18/35） in the Experimental Group and 48, 0% （12/25） in the Control Group, without significant differences （X^2 = 0. 069,P =0. 793）. In the Experimental Group, cag- A positive results were noted in bile samples in 7 cases （20.0% , 7/35 ） and in gallstone samples in 1 case （2.9% , 1/35） , whereas in the Control Group there were no positive results observed. The Hp cag- A positive rate was significantly different between the two groups （X^2 = 5. 822,P = 0. 016）. The presence of Hp cag - A in bile was correlated with the Hp infection of the stomach （X^2 = 3. 886,P = 0. 049）. Conclusions That the DNA of the Hp can be found in bile and gallstones of patients with cholelithiasis indicates a correlation between Hp infection of the gallbladder and the formation of gallstones.