TGF-β1诱导人肾小管上皮细胞血小板反应蛋白1的表达

Influence of TGF-β1 on the expression of thrombospondin 1 in human tubular epithelial cells

目的探讨在TGF-β1诱导下人肾小管上皮细胞(HKCs)血小板反应蛋白1(thrombospondin1,TSP-1)的表达变化。方法实验分二组:对照组(C组)用无血清培养基(FSM)培养人肾小管上皮细胞;TGF-β1组(T组):用含TGF-β120ng/ml的FSM培养HKCs。各组观察24、72和120h3个时相点,分别为C1、C2、C3和T1、T2、T3亚组。用免疫细胞化学及逆转录酶链反应(RT-PCR)方法检测TSP-1及其mRNA的表达的变化。结果T1组比C1组TSP-1表达无显著差异(P>0.05),T2、T3组与C组相应时相点比较TSP-1表达显著增高(P<0.01),T组组内比较随时间延长,TSP-1表达逐渐增高。结论TGF-β1能诱导HKCs表达TSP-1,呈时间依赖性。

Objective To investigate the influence of TGF- β 1 on the expression of thrombospondin 1 （TSP-1） in human tubular epithelial cells, Methods Human tubular epithelial cell （HKCs） line were cultured in vitro with DMEM/F12 （1：1）, HKCs were divided into tow groups： ①Normal control groups （C groups）： HKCs were cultured in free serum medium （FSM）;② TGF - β 1 groups （T groups）： HKCs werecultured in FSM contained TGF- β 1. All cells were redivided into there subgroups C1, C2, C3 and T1, T2, T3 by culturing 24, 72 and 120 hours respectively. The expression of TSP-1 were detected with Immunohistochemistry. The expression of TSP-1 mRNA were analyzed by RT-PCR. The corelation between the relative culture time and TSP-1 mRNA were analyzed, Results TSP 1 expression were negative in C groups and T1 group. However, the expression were positive in T2 group and strong positive in T3 group compared with C2 and C3 groups respectively, P〈0. 01. Statistical analysis showed that there was a positive correlation between culture time and TSP-1 mRNA expression （R = 0.758, P〈0. 01）. Conclusions TGF ~β 1 could time dependenly induce the expression of TSP-1 in HKCs with.