丙型肝炎病毒核心蛋白基因表达及其核苷酸序列分析

Expression of Core Genes of Hepatitis C Virus Genome and Analysis of Their Nucleotide Sequences

以基因扩增及重组DNA技术，从长江下游南通地区急性丙肝和慢性丙肝患者血清中成功地克隆了丙肝病毒核心蛋白基因（HCV－Y1和HCV－Y2）。该段基因在大肠杆菌内已得到高效表达，它们的序列分析发现核苷酸组成的同源性为93．2％，与已报告的HCV株比较属于HCV－Ⅱ型。这对于我国HCV的基因分型以及开发丙肝核心蛋白抗体检测，早期诊断丙肝感染都具有良好的应用前景。

Using polymerase chain reaction and recombination DNA technique,the core gencs(HCV-Y1 and HCV-Y2)of hepatitis C virus genome were cloned in sera from patients with acute-and chronic-hepatitis C in the inshore Nantong area of the Yangtze River.The core genes of HCV were expressed in E.Coli,and highly homeology of their nucleotide sequences were shown to compare with the other isolatcs.They are similar to HCV type Ⅱ like HCV-J and HCV-T.These data should be useful in genetic classification of HCV,detection of antibody to HCV for early diagnosis of HCV infection.