榄香烯对Lewis肺癌小鼠基底膜及细胞外间质影响的实验研究

Experimental Study of Elemene on BM and ECM in Mice with Lewis Lung Cancer

目的:研究基底膜及细胞外基质与肺癌浸润和转移的相互关系,以及中药榄香烯的抗癌作用机理。方法:以C57BL/6近交系小鼠建立Lewis肺癌模型,将40只小鼠分为正常组、模型组、CTX组及榄香烯组,14天后以放免法及SABC免疫组织化学方法,分别检测各组小鼠血清HA、ColⅣ及移植瘤组织LN、FN。结果:榄香烯能降低血清HA、ColIV含量;维持FN在瘤细胞周围基底膜及细胞外基质中的线状表达,降低肿瘤细胞LN表达;与模型组及CTX组比较有显著差别。结论:中药榄香烯与化疗药CTX抗肿瘤作用机理不同,其抑制肺癌转移的部分作用机制是通过保护基底膜和细胞外基质屏障以及降低肿瘤细胞中细胞外基质成分表达而实现的。

Objective： To explore the relationship between the basement membrane （BM） and extracellular matrix （ECM） and the infiltration and metastasis of lung cancer and to explore the mechanism of Chinese herb elemene in anticancer function. Methods： Lewis lung cancer model with C57BL/6 inbreeding line mice was set up. Forty mice were divided into the normal group, model group, CTX group and the Elemene group. The hyaluronic acid （HA） and collagen Ⅳ（colⅣ） in blood serum and the laminin （LN） and fibronectin （FN） in transplatation tumour tissue were measured by the method of radioimmunology and of SABC immunohistochemistry, respectively. Results： In Elemene group, contents of HA and ColIV in blood serum were decreased, lineal shape expression of FN on BM and ECM around tumour cells was maintained, and LN in tumour cells was decreased, which were all significantly different from those in both the model group and the CTX group. Conclusions： The action mechanisms of Chinese herb Elemene are different from those of chemical CTX. The partial action mechanisms of Chinese herb Elemene on inhibiting lung cells metastasis are to protect BM and ECM barrier and to decrease matrix component expression in tumour cells.