摘要
以小麦叶肉细胞原生质体为材料,通过免疫荧光标记和Ca^(2+)荧光染料的装载并结合药物学试验,借助激光共聚焦扫描显微镜观察,探讨微管骨架和Ca^(2+)之间的内在联系。试验结果表明,[Ca^(2+)]_(cyt)的升高能够诱发微管骨架的解聚;而微管骨架的解聚也会促使胞外Ca^(2+)内流,进而造成[Ca^(2+)]_(cyt)的升高。
By using immunofluorescence labeling technique and incubating the Fluo-3AM of Ca^2＋ fluorescein, wheat（Triticum aestivum） cuhivars Lovrin 10 was selected as mesophyll protoplast material in this paper for studying the relationship between the pattern of microtubule and [Ca^2＋]cyt. Laser scanning confocal microscopy（LSCM） was used and pharmacologic examination was set. The results indicated there was a connection between calcium signaling and microtubule organization in wheat mesophyll protoplast. When the cytosolic calcium concentration distinctly ascends,the pattern of microtubule showed depolymerization; Depolymerization of microtubule could enhance extracellar calcium afflux.
出处
《实验生物学报》
CSCD
北大核心
2005年第4期331-339,共9页
Acta Biologiae Experimentalis Sinica
基金
河北省教委博士基金(B2001211)河北省自然科学基金(301124
303180
c2005000220)教育部科学技术研究重点资助项目(03014)植物生理学与生物化学国家重点实验室开放课题资助项目(PPB04006)
