食管鳞癌组织中survivin和caspase-3表达的预后意义及其与热休克蛋白表达的关系

Expression and prognostic significance of survivin and caspase-3 in esophageal squamous-cell carcinoma and their relationship with HSPs expression

目的探讨食管鳞癌组织中survivin和caspase3表达的预后意义及其与HSP27、HSP70表达的关系。方法采用流式细胞术测定临床病理特征相同但术后生存时间明显不同(≤1年和≥5年)的101例食管鳞癌患者肿瘤组织中,survivin和caspase3的表达情况,分析survivin和caspase3表达与食管鳞癌临床病理特征、预后的关系,以及与HSP27、HSP70表达的关系。结果短期生存组食管鳞癌组织中,survivin阳性标记细胞平均荧光强度为6.79±2.11,明显高于长期生存组(5.00±0.77);两组caspase3阳性标记细胞平均荧光强度分别为5.12±0.67和5.07±0.77,差异无统计学意义(P>0.05)。长期生存组survivin强阳性表达率为31.6%,明显低于短期生存组(54.0%,P=0.029);而caspase3在长期生存组的强阳性表达率为68.4%,明显高于短期生存组(47.6%,P=0.042)。caspase3的表达与食管鳞癌患者的年龄、肿瘤长度有关,与其他临床病理特征无关;survivin表达与各临床病理特征均无关(P>0.05)。Logistic回归分析显示,survivin和caspase3表达是食管鳞癌预后的独立影响因素。survivin和caspase3表达与HSP27、HSP70表达无关(P>0.05)。结论survivin和caspase3是食管鳞癌预后的独立影响因素。食管鳞癌组织中survivin和caspase3表达与HSP27和HSP70表达均无关。

Objective To explore the prognostic significance of expression of survivin and caspase-3 in esophageal squamous-cell carcinoma （ESCC） and the relasionship with expression of heat shock proteins 27 and 70 （HSP27 and HSP70）. Methods Expressions of survivin and caspase-3 in 101 cases of ESCC were quantitatively detected with flow eytometry. Their expressions in long-term survival group （group A, ≥5 years, 38 cases） were compared with those in the short-term survival group （group B, ≤1 year, 63 cases）. Their prognostic significance and clinocopathological characteristics were evaluated and their relationship with HSP27 and HSP70 expression was analyzed. Results The mean fluorescence intensity （mFl） of survivin in group B was 6.79± 2.11,which was significantly higher than that （5.00 ± 0.77 ） in group A （ P 〈 0.01 ）. The mFl of easpase-3 in the two groups were similar （5.12 ±0.67 vs. 5.07 ± 0.77, P 〉 0.05）. The positive expression rate of survivin in group A was signitlcantly lower than that in group B （31.6% vs 54.0%, P = 0. 029）. Compared with that in short-term survival group, the strong positive expression rate of easpase-3 in long-term survival group was significantly higher（47.6% vs. 68.4%, P = 0. 042）. Positive expression rate of caspase-3 showed decreasing tendency with increase in age. No significant differences in clinicopathologic features in relation to expression rate of caspase-3 other than tumor length. No correlation was observed between expression intensity of survivin and any clitticopathologic features. Logistic regression analysis indicated that survivin and caspase-3 expressions were of independent prognostic significance for ESCC. There was no association between survivin and caspase-3 expression and expression of HSP27 and HSP70. Conclusion The expressions of survivin and caspase-3 are two independent prognostic factors in ESCC. They do not correlate with HSP27 and HSP70 expression.