摘要
目的:建立高效液相色谱法测定藿阳补肾胶囊中人参皂苷Rg1的含量。方法:采用AlltimaC18色谱柱(4.6×250mm,5μm),乙腈-0.05%磷酸溶液(20:80)为流动相,流速0.8mL·min-1,检测波长为203nm。结果:人参皂苷Rg1在0.39～3.53μg范围内线性关系良好,峰面积与其浓度线性关系良好(r=0.9999),平均回收率99.5%,RSD=0.43%,(n=5)。结论:本法分离效果好、准确、重复性好,可用于该制剂的质量控制。
Objective： To establish method for HPLC deternimation of Ginsenoside Rg1 in Huoyang bushen capsule. Method- The condition of HPLC was Alltima C18 column（4.6×250mm, 5μ m） mobile phase of acetonitrile0.05%phosphoric（20：80） with 0.8mL·min^-1 of flow rate, and the determination wavelength was at 203 nm. Result：The standard curve was linear in the range of 0.3943.53 μg and the correlation coefficient was 0.9999. An average recovery was 99.5%, RSD was 0.43%（n =5）. Conclusion： The method shows good separating result,accuracy and reproducibility, can be used for the quality control of Huoyang bushen capsule.
出处
《中药研究与信息》
2005年第7期18-19,共2页
Research & Information on Traditional Chinese Medicine
