摘要
目的探讨抑癌基因第10号染色体同源丢失性磷酸酶张力蛋白(PTEN)启动子区域甲基化状况与胃癌的关系。方法采用甲基化特异的PCR(MSP)法检测70例胃癌组织及相应癌旁组织中PTEN启动子区域甲基化状态。结果胃癌组织检测到程度不等的甲基化,其中低分化胃癌甲基化发生率为62.5%,与高中分化胃癌甲基化发生率(20.0%、22.2%)有显著性差异(P<0.05)。并且有淋巴结转移的19例胃癌组织中,有12例PTEN基因启动子甲基化,远高于无淋巴结转移组(P<0.05)。癌旁正常组织未检测到甲基化。结论PTEN基因启动子的甲基化与胃癌的发生、转移有关。PTEN甲基化是胃癌患者诊断及预后的候选标志物之一。
Objective To investigate the relationship between methylation status of the 5＇CpG island locating in the promoter region of phosphatase and tensin homolog （PTEN） gene and gastric carcinoma. Methods Using methlation-specific PCR technique （MSP）, specimens from 35 gastric carcinoma patients （tumor tissues, adjacent normal tissues） were detected for methylation status of PTEN promoter region. Results Hypermethylation of the PTEN promoter in gastric carcinoma could be detected effectively, with gastric carcinoma of poorly-differentiated （62. 5 % ） more frequently than high and mediate-differentiated carcinomas. In 19 cases with lymph node metastasis, 12 had PTEN promoter methylation. A significant difference was noted between two groups （P〈0. 05）. In contrast, no methylation occurred in corresponding normal tissues. Conclusion Methylation of PTEN promoter is correlated to oncogenesis, metastasis of gastric carcinoma, and could be used as a putative prognostic indicator for this malignancy.
出处
《华中医学杂志》
2005年第4期263-264,共2页
Central China Medical Journal
