汉坦病毒H8205株MIL-2融合基因的构建

Construction of a fusion gene of Msegement of Hantavirus H8205 strain and IL-2 gene.

目的研究汉坦病毒新型裸DNA疫苗,构建融合基因pcDNA3.1/HisBIL2M(G1+G2)。方法采用PCR扩增汉坦病毒H8205株G1和G2基因片段,将回收的G1和G2片段经双酶切插入到pcDNA3.1/HisBIL2,构建成新的质粒pcDNA3.1/HisBIL2M。结果融合基因转录一分子量为100kD左右的蛋白,对照组则未见电泳条带出现,回收的片段与预期的相符。结论成功构建pcDNA3.1/HisBIL2M融合基因。

Objective To clone a recombinant plasmid containing hantavirus M gene and IL-2 gene for the purpose of developing a new DNA vaccine against hantavirus. Methods The open reading Gland G2 gene fragment of Hantavirus H8205 strain was amplified by using PCR The recycled G1 and G2 fragment were inserted to pcDNA3. 1/His -B-IL-2 which was digested with the same enzymes to construct the recombinant expression plasmid pcDNA3. 1/His-B-IL-2-M. Resnlts The recycled fragment matched the predictive one. Conclusion The fusion gene pcDNA3. 1/His-B-IL-2-M is cloned successfully.