cDNA微阵列结合聚类分析探讨苯中毒免疫相关基因表达谱的改变

cDNA microarray and cluster analysis to identify the significance of immune genes associated with benzene poisoning

目的通过基因表达谱研究寻找苯中毒发病机制中参与苯中毒免疫调控通路的苯中毒免疫相关基因。方法应用含2779条免疫相关基因的cDNA芯片检测7例苯中毒患者和7例正常人的外周血白细胞基因表达谱,观察其在基因表达谱上的差异。进行了基因表达谱聚类分析和比较;筛选有统计学意义的差异表达的免疫相关基因。结果存在差异表达的基因共有38条。在2张以上芯片结果中表达上调的基因10个,主要包括有CD59、TRA@、MCP等。在2张以上芯片结果中表达下调的基因14个,有HLA-DMB、HLA-DQA1、HLA-DPB1、ITGB2、PFC等基因。聚类分析揭示38条基因的基因表达谱特征存在关联。结论通过有统计学意义的差异表达的苯中毒免疫相关基因,推测免疫调控通路在苯中毒发病中可能扮演重要角色。基因芯片技术是分析不同研究对象之间基因差异表达的有效手段。

ObjectiveTo delineate the immune regulatory pathway of benzene poisoning by using gene expression profile analysis. Methods Peripheral white blood cell gene expression profile of 7 benzene poisoning patients, including one aplastic anemia, was determined by microarray. Seven chips from normal workers were served as controls. Cluster analysis of gene expression profile was performed. Differentially expressed immune genes associated with benzene poisoning were determined. Results Among the 2 779 target genes, 38 genes differentially expressed were identified, including 10 up-regulated genes such asCD59,TRA @, MCP etc, and 14 down-regulated genes such as HLA-DMB, HLA-DQA1, HLA-DPB1, ITGB2, PFC etc. Cluster analysis showed that the expression profiles of 38 genes were associated with benzene poisoning. Conclusion Differentially expressed immune genes may play an important role in the pathogenesis of benzene poisoning.