海南岛大珠母贝遗传多样性分析

Genetic diversity of Pinctada maxima(Jameson) germplasm by RAPD analysis

利用RAPD技术首次对中国海南岛环岛的大珠母贝(PinctadamaximaJameson)遗传多性进行分析。从20条10bp引物中选取7条引物用于群体遗传多样性分析,共检测出22个位点,其中11个位点(占50%)显多态性,Shannon多样性值为0.117。用非加权配对算数平均法(UPGMA)聚类分析的结果:12个体间遗传相似系数最大为1.000,最小为0.706,平均遗传相似系数为0.8438。表明海南岛大珠母贝群体间遗传多样性不一,遗传分化较大,因此很有必要对遗传多样性水平较高的种群,划定保护范围加以重点保护,以扩大和恢复大珠母贝的种质资源。

Genetic diversity of Pinctada maxima germplasm from Hainan Islands was studied by RAPD for the first time. Among total 20 10pb primers, 7 were selected for group genetic diversity research. 22 DNA fragments were recognized, in which 11 were polymorphic. The Shannnon Index of diversity of P maxima population was 0.1172. The genetic similarities varied between 1 and 0.706, indicating that the genetic diversities of P maxima inside the population were obviously different from each other. In order to resume the quality of P maxima strain, measures must be taken to protect population of P. maxima that are more polymorphic.