喉癌组织中抑癌基因PTEN的表达及意义

The implications of PTEN expression in laryngeal carcinoma

目的检测抑癌基因PTEN在喉癌组织的表达情况,探讨PTEN与喉癌发生发展之间的关系。方法采用免疫组织化学SABC染色法观察正常喉黏膜和喉癌组织中PTNE蛋白的表达,并比较分析其与肿瘤生长部位、病理分级、临床分期和淋巴转移的关系。结果PTNE在喉癌组织的表达阳性率(52.3%)明显低于正常喉黏膜组织的表达阳性率96.7%,(P<0.05);、期喉癌(71.4%、63.3%)PTEN蛋白表达阳性率又明显高于、期喉癌(25.0%、22.2%),(P<0.05);声门型喉癌(65.8%)明显高于声门上型和声门下型喉癌(34.8%、25.0%),(P<0.05);高分化喉鳞癌(88.0%)明显高于低分化喉鳞癌(37.5%、18.8%),(P<0.05);N0喉癌(78.4%)明显高于N1、2喉癌(17.9%),(P<0.05)。结论PTNE在喉癌的发生及发展过程中有表达缺失,缺失程度与临床分期、生长部位、病理分级和淋巴转移有相关性。

Objective To investigate the implications of expressive activity of tumor suppressing gene PTEN in the tissues of laryngeal carcinoma（LC）. Methods Sixty-five cases with LC and 30 cases with nodules or polyp of vocal cord were included in this study, taken as observing group or controlling one respectively. The techniques utilized here were immunohistochemical SABC procedures to detect the expression of PTEN in paraffin-embedded tissue samples of these cases. Then, an analysis was made to explore the association of PTEN expression with the features of clinical pathology among them. Results The positive expressing rate of PTEN was significantly lower in LC tissues（52.3%） than that in begin laryngeal lesions （96.7%）, P〈0.05. Furthermore, its expressing rate was significantly higher among the cases with the primary loci of LC at stages of T1（71.4%） or T2（63.3%） than those at stages of T3 （25.0%） or T4（22.2%） respectively,P〈0.05. This rate was also significantly higher among the cases with glottic type of LC（65.8%） than those with supra-or hypo-glottic type of LC（34.8% and 25.0% respectively）,P〈0.05. The similar tendencies of implications were true for the associations of PTEN expression with the level of differentiation and the metastatic condition of neck lymph nodes as well. Conclusions The level of PTEN expression should be involved in the process of laryngeal tumorogenesis and LC developing and is associated with the clinicopathologicat features of this tumor.