VEGF,KDR,MMP-1及转录调节因子Ets-1在卵巢癌组织中表达

Expression and Significance of VEGF, KDR, MMP-1 and Transcription Factor Ets-1 in Ovarian Carcinoma

[目的]从分子水平观察肿瘤血管生成相关因子VEGF、VEGF受体KDR、基质金属蛋白酶1(MMP-1)以及转录调节因子Ets-1在卵巢上皮性癌中表达规律并揭示其因子间相关关系,为解释促血管生成因子间内在规律提供依据。[方法]利用原位杂交和免疫组织化学染色法检测87例卵巢上皮性癌中VEGF、KDR、MMP-1以及Ets-1等因子mRNA和蛋白表达规律。[结果]VEGFmRNA和蛋白主要分布在癌细胞胞浆中,阳性率分别为77.1%(67/87)和70.1%(61/87);KDRmRNA和蛋白主要在内皮细胞上表达,其表达率与VEGF表达率间存在密切的相关关系(r=0.892);MMP-1在肿瘤细胞和间质血管内皮细胞中表达,尤其在血管新生处表达明显;Ets-1主要分布在内皮细胞中,其表达率与KDR和MMP-1表达率间相关系数分别为0.9004和0.873。[结论]VEGF、KDR、MMP-1以及Ets-1是参与卵巢上皮性癌血管生成的重要因子,为抗肿瘤血管治疗新的治疗靶点。

[Purpose] To investigate the expression of VEGF, KDR, MMP-1 and its transcription factor Ets-1 on the interstitial neovasculogenesis in human epithelial ovarian carcinoma on molecular level, which may be provides further theoretic basis for explain interregularity between neovasculagenetic factors. [Methods] VEGF, KDR, MMP-1 and Ets-1 in 87 cases with epithelial ovarian carcinomas were detected by in situ hybridization and immunohistochemistry method. [ Results ] VEGFmRNA and its protein were mainly expressed in cytoplasm of cancer cells, and positive rates were 77.1% （67/87） and 70.1% （61/87 ） respectively. KDRmRNA and its protein were mainly expressed in endothelial cells, corrlated with VEGF expression （r=0.892）.Over expression of MMP-1 was seen in both endothelial cells and tumor cells, especially in hyperplasia of endothelial cells. Ets-1 was mainly expressed in endothelial cells, and correlation coefficient between Ets-1 and KDR, MMP-1 were 0.9004 and 0.873 respectively. [Conclusion] VEGF, KDR, MMP-1 and Ets-1 are important factors of neovasculogenesis in human epithelial ovarian carcinoma, which may be provides new therapeutic target in antineovasculagenetic therapy.