人微量全血培养与染色体标本制作技术研究

The study of whole micro-blood culture and the techniques of making chromosome specimens from Human body

采用微量全血培养技术,培养人体外周血小淋巴细胞,使G0期白细胞重新进入细胞周期、增殖成功率高、技术方法完善。把体外增殖的小淋巴细胞裂解,对细胞核染色体进行染色和固定,制得人染色体标本,用于进行染色体组型分析,取得了令人满意的效果。

The well - established techniques of whole micro - blood culture method were applied to breed Human body outer lane small lymphocytes. By this way, the GO phase leukocytes can enter cell cycle again and is of high proliferation.The results were obtained by splitting the multiplying small lymphocytes dyed and fixed the leukocyte cell nucleus which were used for chromosome analysis.