COX-2在皮肤鳞状细胞癌中的检测

Detection of COX-2 in Squamous Cell Carcinoma

目的探讨环氧化酶-2(COX-2)在皮肤鳞状细胞癌中的水平及与增殖指标K i67的关系。方法应用免疫组化SP染色法对57例皮肤鳞状细胞癌皮损及20例正常皮肤组织石蜡切片进行COX-2,K i67染色,在光镜下观察并计数其阳性细胞。结果①COX-2与K i67在正常皮肤组织中均无阳性染色,在皮肤鳞状细胞癌皮损中染色强度显著增高(P<0.05);②分化程度不同的皮肤鳞状细胞癌皮损中COX-2蛋白强弱差异无显著性(P=0.756),而K i67有显著性差异(P<0.01);③COX-2与K i67在皮肤鳞状细胞癌皮损中的水平呈显著正相关(r=0.767,P=0.05)。结论在皮肤鳞状细胞癌中COX-2,K i67表达异常增高,二者呈正相关;过度表达的COX-2蛋白可能参与肿瘤细胞的增殖过程。

Objective To investigate the significance and relationship of cyclooxygenase-2 （COX-2） and Ki67 in the pathogenesis and development of squamous cell carcinoma （SCC）. Methods Specimens from 57 SCC patients and 6 normal persons were assayed for COX-2 and Ki67 using immunohistochemistry. Results ①No COX-2 and Ki67 in normal specimens was detected, but it was significantly increased in SCC （ P 〈 0.05 ）. ②The COX-2 showed no correlated with the tumor differentiation in SCC （P = 0.756） ,but Ki67 was significantly different in SCC of different histological grade（ P=0.002）. ③ COX-2 was positive correlated with Ki67 （P = 0.05 ）. Conclusion The abnormal level of COX-2 and Ki67 plays a role in carcinogenesis and development of SCC, the over - expression of COX-2 may result in the proliferation of tumor cells.