牛摩拉氏菌溶血素基因克隆的探讨

CLONING AND EXPRESSION OF THE MORAXELLA BOVIS HEMOLYSIN STRUCTURAL GENE IN ESCHERICHIA COLI

应用分子遗传学方法，以光敏生物素标记的ＤＮＡ探针进行分子杂交等手段克隆了牛摩拉氏菌（Ｍｏｒａｘｅｌｌａｂｏｖｉｓ）溶血素结构基因，并在大肠杆菌中得到表达，其重组子定名为ＭＲ９，分子量为６．４５Ｋｂ，外源片段分子量为３．８Ｋｂ。

With Moraxella bovis strain FLA64 as a donor and plasmid pUC18 as a vector, the hemolysin structural gene of M. bovis FLA64 was cloned and expressed in E.coli DH5α which was deficent in hemolysin gene.The recombined plasmid MR9 which contained the cloned homolysin structural gene was identified by expressing of hemolytic activity and hybridization of photobiotin probles of pUC18and FlA64. It has been found that this cloned gene was located on about 3.8 Kb Hind Ⅲ-EcoRⅠ fragment.