摘要
由东乡野生稻的细胞悬浮培养物游离原生质体,采用改良的RY-2培养基以琼脂糖珠法进行包埋培养,能产生持续细胞分裂。预分化实验表明:ABA能有效地提高原生质体愈伤组织的再生能力。最后成功地诱导出原生质体再生植株。
Protoplasts isolated from cell suspension cultures of Dongxiang Wild Rice were cultured in modified RY-2 medium with agarose-ball embedding culture method. Predifferentiation showed that ABA was effective to increase the frequency of regeneration of protoplast-derived calli.Regenerated plantlets were achieved in the wild species of Orysa.
出处
《江西科学》
1995年第3期176-179,共4页
Jiangxi Science
基金
江西省科委资助
江西省自然科学基金
关键词
东乡野生稻
原生质体
再生植株
野生稻
Dongxiang Wild Rice, Protoplast, Predifferentiation, Regenerated plantlets