反相高效液相色谱法测定菊黄上清含片中甘草酸含量

Content Assay of Glycyrrhizic Acid in Juhuangshangqing Buccal Tablets by RP-H PLC

目的:建立菊黄上清含片中甘草酸的含量测定方法。方法:采用高效液相色谱法,色谱柱为HypersilC18柱(250mm×5.0mm,5μm),流动相为乙腈-1%醋酸(35∶65),流速为1mL/min,检测波长为250nm。结果:甘草酸的进样量在0.43～6.9μg范围内与峰面积呈良好的线性关系(r=0.9999),平均加样回收率为99.73%,RSD为1.75%(n=9)。结论:该方法准确、简便、快速,具有实用性,可用于菊黄上清含片的质量控制。

Objective：To establish assay method of the content of glycyrrhizic acid in Juhuangshangqing buccal tablets. Methods：By RPHPLC,column is Hypersil C18（250 mm×5.0 mm,5μm）,mobile phase is acetonitrile-1% acetic acid（35：65）,flow-rate is 1.0 mL/min, detection wavelength is 250 nm. Results：The injection sample dose of at 0. 43-6.9μg, it has well linear relation with peak area（r = 0. 999 9）, average recover rate is 99.73%, RSD is 1.75%.Conclusion：RP-HPLC is convenient and easy to perform, it is accurate and reliable,is applicable to quality control of Juhuangshangqing buccal tablets.