摘要
目的:建立抗饥消渴片的质量标准。方法:采用薄层色谱法(TLC法)对处方中的黄连、黄柏、红参进行定性鉴别;采用高效液相色谱法(HPLC法)对黄连、黄柏中的盐酸小檗碱进行含量测定。色谱条件为PhenomenexC18柱(250mm×5mm,5μm),流动相为用磷酸调节pH=7的乙腈-0.03mol/L磷酸二氢钾溶液-1%三乙胺溶液(31∶59∶10),流速为1.0mL/min,检测波长为265nm。结果:在TLC图谱中可检测出黄连、黄柏、红参的特征斑点。盐酸小檗碱线性范围为0.0624~0.2808μg,平均回收率为98.34%,RSD=0.74%(n=6)。结论:方法简便、准确,可用于抗饥消渴片的质量控制。
Objective: To establish a quality standard for Kangjixiaoke tablets. Methods:Rhizoma coptidis, cortex Phellodendri, Radix Ginseng Rubra were identified by TLC, and the content for berberine hydrochloride in Kangjixiaoke tablets was determined by HPLC. Condition of HPLC was: Phenomenex C18 column (250 mm ×5 mm, 5μm); the mobile phase was a mixture of acetonitrile-0. 03 mol/L phosphoric acid- 1% triethylamine(31 : 59 : 10); the flow rate was 1, 0 mL/min;the detection wavelength was 265 nm. Results:Rhizoma coptidis, cortex Phellodendri, Radix Ginseng Rubra could be identified by TLC. Berberine hydrochloride showed a good linear relationship at a range of . 0624 -0. 2808μg The average recovery was 98.34% with RSD 0. 74% (n=6), Conclusion: The method is simple,accurate and can be used for quality control of Kangjixiaoke tablets.
出处
《中国药业》
CAS
2005年第8期24-25,共2页
China Pharmaceuticals
关键词
抗饥消渴片
黄连
黄柏
红参
盐酸小檗碱
质量标准
Kangjixiaoke tablets
Rhizoma coptidis
cortex Phellodendri
Radix Ginseng Rubra
berberine hydrochloride
quality standard