摘要
目的:测定消癌平片中绿原酸的含量。方法:高效液相色谱法(HPLC法),色谱柱为C18柱,流动相为乙腈-水-冰醋酸(8∶92∶1),流速为1.0mL/min,检测波长为327nm。结果:线性范围是0.0816~0.4080μg,r=0.9999,平均回收率为99.09%,RSD=1.25%(n=6)。结论:HPLC法简便、准确、重现性好,可作为消癌平片中绿原酸的含量测定方法。
Objective: To establish a HPLC method for determing the content of chlorogenic acid in Xiaoaiping tablets. Methods: The content of Xiaoaiping tablet was determined by HPLC. The analysis was carried on C18 column,the mobile phase was consisted of acetonitrile-puritied water-acetic acid(8:92 : 1),the flow rate was 1.0 mL/min,and the utlraviolet detection wavelength was at 327nm, Results:The linear regression for Xiaoaiping tablet was over the range of 0.081 6-0.408 0μg(r=0.999 9).The average recovery rate was 99.09% with RSD 1.25% (n =6). Conclusion: This method was sensitive,accurate,reproducible and exclusive. It can be used for quality control of Xiaoaiping tablets.
出处
《中国药业》
CAS
2005年第8期26-27,共2页
China Pharmaceuticals