摘要
目的:建立板蓝根中精氨酸的含量测定方法。方法:采用高效液相色谱法(HPLC法)测定。结果:精氨酸的浓度在48~240μg/mL范围内与峰面积线性关系良好,r=0.9995;平均回收率为99.55%,RSD=0.73%。结论:HPLC法操作简便、准确,可作为板蓝根中精氨酸的含量测定和质量控制方法。
Objective: To establish the method for determining the content of arginine in Isatis indigotica Fort. with HPLC. Methods: HPLC method was selected to determine the content. Results:The linear range of calibration curve was 48-240μg/mL for the arginine(r =0. 999 5). The average recovery was 99.55% and the RSD was 0.73%. Conclusion: The method is simple, and accurate with a good reproducibility and can be used as quantitative analysis for this preparation.
出处
《中国药业》
CAS
2005年第8期29-30,共2页
China Pharmaceuticals