国内部分地区松材线虫和拟松材线虫基因多态性的RAPD分析

Genetic Assay of Bursaphelenchus xylophilus and B. mucronatus from Some Plague Pot of China by RAPD

采用SDS法提取线虫基因组DNA,分别选取了6种随机引物对部分地区的10株松材线虫和10株拟松材线虫进行随机扩增和亲缘关系比较。RAPD的结果可以明显区分松材线虫和拟松材线虫,与形态学分类相吻合。聚类分析的结果也表明,线虫种内和种间均存在差异,但种间差异明显大于种内差异,同种线虫在同一地区或临近地区的同源性明显高于其它地区,同一地区内寄主对线虫种类分布的影响较小。该方法具有所需样品量少、检测灵敏度高、结果准确等优点,为松材线虫种群的亲缘关系、传播途径及其检测防疫等方面提供一种灵敏、可靠的研究方法。

After extracting Bursaphelenchus xylophilus and B. mucronatus genomic DNA by SDS method, ten strains of B.xylophilus and ten strains of B. mucronatus from some epidemic areas were compared in the relationships by selected six primers through RAPD assay. The result of RAPD could discern obviously B. xylophilus from B. mucronatus and the result is consistent with morphology analysis. The cluster analysis also demonstrated between interspecific and intraspecific variations, which interspecific variations were more than intraspecific variations. Nematode homology in the same and adjacent district is higher than in distant district. In the same district host has very little effect on the nematode distribution. The method is sensitive and accurate, and can be regarded as a reliable technique for the study of nematode distribution, spread pathway, detection and prevention.