不同形式硒对大鼠生长及短期肝癌模型发生的影响

Effect of Different Formality Selenium on Rat Growth and the Progress of Short-term Hepatocarcinoma Model in Rats

SD大鼠75只,体重50～60 g,随机均分5组.组Ⅰ,正常对照组;组Ⅱ,试验对照组;组Ⅲ,高亚硒酸钠组;组Ⅳ,富硒麦芽组;组Ⅴ,富硒酵母组.组Ⅰ、Ⅱ、Ⅲ各组基础饲料中分别添加亚硒酸钠硒,含量分别为0.1、0.1、0.3mg/kg;组Ⅳ,基础饲料中添加富硒麦芽硒0.3 mg/kg;组Ⅴ,基础饲料中添加富硒酵母硒0.3 mg/kg.各组饲喂基础料第32天时,除组Ⅰ大鼠外,其余4组大鼠实施'AFB1致肝癌短期实验模型程序'.记录各组大鼠体重情况,测定血清碱性磷酸酶(ALP)、甲胎蛋白(AFP)、NO和γ-谷氨酰转移酶(γ-GTase)、SOD、MDA含量,以及肝、肾组织和全血中谷胱甘肽过氧化物酶(GSH-Px)、MDA、SOD、羟脯氨酸(HyP)的活性.结果:与对照组相比,富硒酵母硒和富硒麦芽硒能显著促进大鼠生长,提高大鼠增重率、降低料肉比,而亚硒酸钠硒作用不明显;并明显降低血清γ-GTase、HyP水平,提升全血GSH-Px活性及血清SOD水平,有效清除肾脏MDA;在降低血清ALP、AFP和快速提高全血GSH-Px活性方面,富硒麦芽硒作用优于富硒酵母硒.表明富硒酵母硒和富硒麦芽硒比亚硒酸钠硒能更有效提高大鼠生长速度,增强其抗氧化能力,从而抵抗AFB1所致肝肿瘤.

75 SD rats were used and randomly divided into 5 groups of 15 rats,including 8 male and 7 female each. The selenium contents in feed of group Ⅰ, Ⅱ and Ⅲ were 0.1, 0.1 and 0.3 mg/kg from sodium selenite respectively. 0.3 mg/kg selenium from Se-enriched malt and Se-enriched yeast were supplemented to the diet of group Ⅳ and Ⅴ respectively. After 32 days, rats of group Ⅱ, Ⅲ,Ⅳ and Ⅴ were treated with the program, which named short-term animal hepatocarcinoma model, by aflatoxin B1 （AFB1）. Changes of feed intake, body weight, the haemal-indicators, such as alkaline phosphates（ALP）, alpha-fetoprotein （AFP）, nitric monoxide （NO）,γ-glutamyltranspeptidase （γ-GTase）, superoxide dismutase （SOD） and malonadehyde （MDA） in serum, the antioxidative indices, such as glutathione peroxidase （GSH-Px）, SOD and MDA in blood and tissue, the activity of hydroxyproline（HyP） in liver were measured. The data showed that effect of selenium from Se-enriched malt and yeast were better than sodium selenite in the aspects of body weight gaining, raising GSH-Px and SOD activity, clearance of kidney MDA and decreasing the activity of HyP and γ-GTase. The selenium from Se-enriched malt was better than that of yeast by raising rapidly GSH-Px in blood and decreasing the activity of HyP and γ-GTase. In conclusion, the effect of selenium from Se-enriched malt and yeast on the hepatocarcinogenesis induced by AFB was better than that of sodium selenite in stimulating the antioxidative function and stimulating the rat growth.