内皮素-1对肉鸡腹水综合征发生发展的影响

Effect of Endothelin-1 on the Formation and Development of Ascites Syndrome in Broilers

利用放射免疫分析方法,分别测定了35日龄正常组、轻度腹水组、重度腹水组肉鸡血浆内皮素-1(ET-1)、心钠素(ANP)的含量以及心、肝、肺、肾等组织ET-1的含量,并测定了腹水心脏指数(AHI)、平均右心室压(mRVP)和平均肺动脉压(mPAP);同时还用硝酸还原酶法测定了血清中一氧化氮(NO)的水平。结果发现,随腹水程度的加深,mRVP、mPAP、AHI极显著(P<0.01)升高,肉鸡血浆ET-1、ANP和组织中ET-1的含量以及血清NO浓度也显著(P<0.05)或极显著(P<0.01)升高,且重度腹水组高于轻度腹水组;另外还发现,血浆ET-1、ANP与mPAP、AHI以及心脏ET-1含量之间相关性显著(P<0.05),而血清NO含量与上述指标之间无明显的相关性(P>0.05)。结果表明ET-1、ANP、NO均参与了肉鸡AS的发生和发展过程并在其中起着重要作用,其中ET-1、ANP与肉鸡发生腹水的程度有密切的关系。

Radioimmunoassay was used to detect endothelin-1 （ET-1）, atrial natriuretic polypeptide （ANP） in plasma and ET-1 in heart, liver, lung and kidney of normal group, light ascites group and heavy ascites group of 35-days old commercial broilers. The AHI, mRVP and mPAP were measured as well. At the same time, nitrate reductase was used to detect the level of nitric oxide （NO） in serum and AHI was measured as well. It was found that, compared with the normal group, the AHI, mRVP and mPAP of AS groups were significantly higher, and the heavy ascites group was significantly higher than that of the light ascites group, the trend of increment of them was observed following the degree of ascites. Other indexes such as the level of ET-1 in plasma and tissues, ANP in plasma, NO in serum have the same tendency. The level of ET-1 of tissues was higher than that of plasma and the level of ET-1 of lung and heart was significantly higher than that of other organs. Besides, there was a significant positive correlation among plasma ANP, plasma ET-1, mPAP, heart ET-1 and AHI, but no significant correlation was found between serum NO and other indexes among the three groups. It is concluded that ET-1, ANP and NO took part in the formation and development of AS in broiler and played an important role in it.