摘要
目的:探讨原发性肾小球肾炎(PGN)Th1/Th2细胞因子失衡情况,以及不同病理类型、不同病程对Th1/Th2失平衡的影响。方法:采用酶联免疫吸附(ELISA)法测定16例正常人及38例PGN患者血浆IL-18及IL-13水平,同时应用免疫组织化学检测6例正常肾组织和38例PGN患者肾组织IL-18及IL-13的表达量。结果:无论在肾组织抑或外周血,PGN患者IL-18水平及IL-13水平均较正常对照组显著上升(P均<0.001),但肾组织抑或外周血IL-18/IL-13比率与正常对照组比较无统计学差异(P>0.05);在肾组织局部,除膜增生性肾小球肾炎(MPGN)患者IL-18/IL-13显著高于正常对照组(P均<0.001)外,其他病理类型PGN患者IL-18/IL-13比率与正常人比较无统计学差异(P>0.05),各病理类型PGN患者血浆IL-18/IL-13比率与正常对照组比较也无统计学差异;虽然外周血及肾组织IL-18及IL-13水平与血清肌酐(Scr)水平具有密切正相关关系,但IL-18/IL-13比率与Scr水平无相关关系(P>0.05)。结论:除极个别病理类型外,PGN患者免疫紊乱状态似乎不能简单的按Th1优势/Th2优势进行二分法分类,其免疫紊乱状态远较此复杂,试图通过简单的调节Th1/Th2平衡来治疗PGN似乎仍缺乏坚实的理论及实验基础。
Objective：To investigate the state of TH1/TH2 cytokines imbalance in patients with primary glornerlonephritis（PGN） and the influence of pathological types and disease stages on TH1/TH2 imbalance state. Methods. Plasma levels of interleukin- 18（IL- 18） and interleukin- 13（IL- 13） in 38 patients with PGN and those of 16 normal controls were measured by enzyme - linked immunoabsorbent assay （ELISA）. The expression of IL - 18 and IL - 13 in the renal tissues from all the subjects were also detected by immunohistochemical assay. The ratio of plasma and renal IL- 18/IL- 13 was then calculated. Results： Plasma levels and renal expression of IL- 13 and IL- 18 in patients with PGN were increased significantly when compared to those of normal controls（P 〈 0. 001 respectivdy）. The ratio of plasma and renal IL- 18/IL-13 was not different significantly in patients with PGN when compared to that of normal controls. The ratio of renal IL- 18/IL - 13 was increased significantly in patients with membrane p mliferative glomerulonephritis （MPGN） （ P 〈 0.001 ）. The ratio of plasma and renal IL- 18/IL- 13 ratio was not correlated significantly with the levels of serum creatinine （Scr） though the levels of IL- 18 and IL - 13 alone were correlated with the levels of Sex. Conclusion： It seems that the immune disturbance state of PGN can not be simply divided into Thl predominant and Th2 predominant except a few pathological types,whichseern far more complicated than this Th1 / Th2 paradigm. More evidence should be found before using regulation of Th1/Th2 balance to treat the diseases.
出处
《中国中西医结合肾病杂志》
2005年第8期453-456,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
深圳市宝安区科技局基金资助项目(No.2002047)
