核转录因子κB在门静脉高压大鼠肺血管中的表达及意义

Expression of NF-κB in Pulmonary Vessels of Rats with Portal Hypertension and Its Implications

目的:探讨核转录因子-κB(NF-κB)在门脉高压大鼠肺血管中的表达及意义。方法:建立门脉高压大鼠模型,将实验动物随机分为三组:实验模型组,银杏治疗组,对照组,动态观察其肺血管NF-κBp65的表达。结果:对照组平均光密度值0.2229±0.0008、实验组0.3007±0.0044、治疗组0.2866±0.0037。造模后第2周、3周、4周,实验组为:0.2739±0.0383、0.2982±0.0754、0.3202±0.0592;治疗组为:0.2625±0.0534、0.2890±0.0686、0.2918±0.0683。对三组结果进行两两非配对t检验,都具有显著性差异(P<0.01);相同时间段实验组与治疗组间亦具有显著性差异(P<0.01)。结论:门脉高压可促成肺血管组织NF-κBp65的高表达,并在门脉高压引起的血管病变中发挥重要作用;银杏注射液对NF-κBp65的高表达有抑制作用。

Objective： To investigate the expression of NF-κB in pulmonary vessels of rats with portal hypenension（PH） and its implications. Method： Rats were randomly allocated to three groups： ①Experimental group; ②Treatment group; ③Control group. Detected the expression of NF-κB by immunohistochemistry. Results： There are significant difference between each two groups （P〈0.01）, even during the same period of time there are significant difference too（ P〈 0.01 ）. Conclusion： PH can cause over expression of NF-κB in pulmonary vessels of rats. NF-κB may play an important role in PH vasculopathy. Therapeutic treatment of Gingko injection can inhibne over-expression of NF-κB p65 induced by PH.