妊娠高血压综合征患者vWF、t-PA、PAI-1的检测分析

Detection and Clinical Significance of vWF,t-PA and PAI-1 in Patients with Pregnancy Induced Hypertension

目的:探讨妊娠高血压综合征(妊高征)患者内皮细胞功能指标的变化及其临床意义。方法:应用ELISA法及发色底物法测定妊高征患者血浆血管性血友病因子(vWF)、织织型纤溶酶原激活物(t-PA)及纤溶酶原激活抑制物-1(PAI-1)活性。结果:妊高征患者vWF、t-PA、PAI-1较正常非孕组明显升高(P<0.05或P<0.01);妊高征各组患者vWF、PAI-1活性比正常晚孕组显著增高(P<0.05或P<0.01),且病情越重增高越明显;t-PA无明显改变。中、重度妊高征组血浆vWF与PAI-1水平呈直线正相关关系(r=0.723,P<0.05;r=0.765,P<0.05)。结论:妊高征患者内皮细胞功能异常,凝血及纤溶抑制功能亢进。测定血浆vWF和PAI-1水平,对于临床诊断妊高征有重要意义。

Objective： To explore the changes and clinical significance of plasma von willebrand（vWF）, tissue-type plasminogen activator（t-PA） and plasminogen activator inhibitor-1 （PAI-1）levels in patients with pregnancy induced hypertension（PIH）. Method： Plasma vWF, t-PA and PAI-1 were determined by ELISA and chromogenic assay respectively in 50 non-pregnancy women（control group）, 50 normal pregnancy women（normal pregnancy group）and 85 patients with PIH（PIH group）. Results： In normal late pregnancy group and PIH group,vWF,t-PA and PAI-1 are significently higher than those in control group; In PIH group, vWF and PAI-1 are significently higher than those in normal pregnancy group, but t-PA does not. There is a positive correlation between the plasma vWF and PAI-1 levels in moderate and severe PIH patients. Conclusion： There is endothelial damages in patients with PIH. Observing the levels changes of plasma vWF and PAI-1 is important for the diagnosis and therapy of PIH.