中国湖北地区汉族人群P-选择素基因-2123C/G多态性分布的研究

Study on Distribution of P-selectin Gene -2123C/G Polymorphism in the Human Crowd of Hubei Area

目的:探讨P-选择素(P-selectin)基因启动子-2123位点多态性在中国湖北地区汉族正常人群中的分布特点。方法:采用聚合酶链反应(PCR)及限制性片断长度多态性(RFLP)方法检测中国湖北地区170名汉族人群P-选择素基因启动子-2123位点多态性,探讨其基因型及等位基因分布特点。结果:中国湖北地区人群P-选择素基因启动子-2123位点各基因型频率CC型8.20%,CG型37.60%,GG型54.10%。等位基因频率C为27.10%,G为72.90%。与欧洲国家人群相比,该位点多态性存在显著差异(P<0.01)。结论:P-选择素基因启动子-2123位点多态性有明显的种族差异。

Objective: To observe the distribution of P-selectin gene -2123C/G polymorphism in the normal human crowd of Hubei area,China, and analyze distribution difference of this polymorphism among different races.Method: The frequency of P-selectin genotypes and alleles were detected by using the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 170 normal Chinese Hans in Hubei area.Results: In Hans crowd of the area Hubei,China,the frequency of P-selectin genotype GG is 8.20%, GC is 37.60%, CC is 54.10%, respectively. The frequency of major G allele is 27.10%, and C is 72.90%. Compared with American and European population, the polymorphism are significantly different(P<0.01). Conclusion: The polymorphism of the P-selectin gene -2123C/G are varied significantly among races.