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人表皮生长因子受体2抑制剂联合白细胞介素-2对人肾癌细胞株的疗效观察 被引量：3

Influence of trastuzumab with IL-2 on HER2 and MRP1 of ACHN
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摘要 目的:了解曲妥珠单抗(Trastuzumab,商品名为赫赛汀)联合白细胞介素-2(IL-2)对肾癌细胞在体外的杀伤抑制作用,同时检测肾癌细胞在药物作用前后人表皮生长因子受体2(HER2)、多药耐药相关蛋白1(MRP1)的表达情况。方法:肾癌细胞系通过细胞培养技术进行培养,用单四唑(MTT)法观察曲妥珠单抗联合IL-2对该细胞系的抑制杀伤效果,进而运用链霉抗生物素-过氧化物酶免疫组化(S-P)法对HER2、MRP1表达进行测定。结果:曲妥珠单抗联合IL-2对肾癌细胞的生长抑制及耐药性呈时间和剂量依赖性。经药物处理后,HER2、MRP1表达明显下降(P<0.05)。结论:曲妥珠单抗能有效地抑制肾癌细胞的生长,对瘤细胞的耐药性也有一定的逆转作用。 Objective： To study the influences of Trastuzumab and IL-2 on human epidermal growth factor receptor-2 （ HER2）, muhidrug resistance-associated protein 1 （ MRP1 ） of ACHN in vitro. Methods ： ACHN cell line of RCC were cultured by cell culture technique. The tetrazolium-based colorimetric assay was used to evaluate the growth inhibitory effects of trastuzumab and IL-2. S-P method was used to determine the expression of HER2, MRP1 of the cells. Results： Trastuzumab showed the inhibitory effects on growth and multi-drug resistance in RCC from 40μg/L or 24 h in time-effective and dose-dependent manner. After treatment with Trastuzumab and/or IL-2, the expression of HER2, MRP1 genes of RCC was decreased significantly（ P 〈 0.05 ）. Conclusion： Trastuzumab and IL-2 can inhibit the proliferation of RCC and decrease the expression of HER2, MRP1 in ACHN.
作者 王家菁 胡志全 叶章群
机构地区 华中科技大学同济医学院附属同济医院泌尿外科
出处 《医学研究生学报》 CAS 2005年第8期686-689,共4页 Journal of Medical Postgraduates
基金 华中科技大学同济医学院科研基金资助项目(批准号:200449)
关键词 人肾癌细胞株 白细胞介素-2 人表皮生长因子受体2 多药耐药相关蛋白1 Human caucasian kidney adenocarcinoma Interleukin-2 Human epidermal growth factor receptor2 Multidrug resistance-associated protein 1
分类号 R392.11 [医药卫生—免疫学]
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参考文献6

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二级参考文献19

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同被引文献31

  • 1肖争,王学峰,晏勇,栾国明,阎丽.难治性癫癇患者脑组织中5种耐药基因产物的同步表达及临床意义[J].中华神经科杂志,2004,37(6):500-503. 被引量：17
  • 2魏虎来,葛建国,赵怀顺,王东海,白德成.干扰素和环孢霉素A协同逆转K562/ADM细胞对阿霉素的耐药性[J].肿瘤防治研究,2005,32(2):99-101. 被引量：10
  • 3平宝红,周淑芸,刘启发,杨纯正.干扰素逆转白血病细胞多药耐药性的研究及机制的初步探讨[J].中华血液学杂志,1996,17(1):13-15. 被引量：15
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引证文献3

二级引证文献7

医学研究生学报
2005年 第8期

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