托吡酯促进高血压鼠脑缺血再灌注损伤后热休克蛋白70表达的研究

Effect of topiramate on Heat Shock Protein 70 expression after cerebral ischemia and reperfusion injury

目的观察托吡酯对高血压大鼠急性脑缺血再灌注损伤后热休克蛋白70(HSP70)表达的影响,探讨其神经保护作用机制。方法Wistar大鼠行双肾动脉狭窄术制成高血压模型,喂养2个月后随机分为3组:托吡酯干预组、缺血再灌注组和假手术对照组。线栓法制做大脑中动脉闭塞(MCAO)模型,缺血2h,再灌注1d。取脑组织切片经免疫组化染色后,用病理图象分析仪计数HSP70阳性和阴性细胞数并计算阳性细胞比。结果托吡酯干预组大鼠HSP70的表达高于缺血再灌注组(P<0.05),假手术对照组未见HSP70表达。结论托吡酯能促进HSP70的表达,对缺血再灌注损伤的脑组织有保护作用。

Objective To study the effect of topiramate on the expression of Heat Shock Protein 70 （HSP70 after brain ischemia and reperfusion injury. Methods Wistar rats were performed operation of double renal artery stenosis to make hypertension. 2 months after, the rats were separated into 3 groups randomly： topiramate group,ischemic reperfusion group and fake operation control group. The rats were performed operation of middle cerebral artery occlusion （MCAO） and made them in ischemia for 2 h. One day later, their brains were obtained, and the number of positive and negtive cells were counted by computerized pathological image analyzer. Results The HSP70 expression of topiramate group was higher than that of ischemic reperfusion group（P〈0.05）. No positive cell had been found in the samples of the control group. Conclusion Topiramate can enhance the expression of HSP70 and may therefore protect the ischemic brain tissue from reperfusion iniury.