针刺治疗缺血性脑损伤的实验研究

Effect of electroacupuncture on the cerebral ischemia in rats

目的探讨电针对大鼠急性脑梗死后神经可塑性影响的物质基础及其发生机制。方法采用线栓法闭塞大鼠大脑中动脉,制备局灶性脑缺血模型,用光镜、电镜、免疫组织化学方法观察电针“前三里”、“后三里”穴对大鼠梗死区域缺血损伤组织、坏死神经元和血管、突触数目等变化的影响。结果电针可以改善组织病理学指标,促进突触数目和结构的恢复,增加MAP2和SYN的表达。结论电针对局灶性脑缺血Wistar大鼠有明显的保护作用,促进脑缺血后突触功能的重建。

Objective To investigate the effect of electroacupuncture on the structure basis of nervous plasticity and the recovery mechanism after acute cerebral infarction. Methods A rat model of focal cerebral ischemia was made by filament occlusion of the middle cerebral artery. The effect of electroacupuncture in Qiansanli and Housanli to a rat＇s change in infarction tissue, necrosis of neuron and vessel, and the number of synapse. Results Electro acupuncture could improve histopathologic indices, increase the number of synapse, and promote the recover of synapse＇s structure. Conclusion Electro acupuncture can produce a marked protecting effect on the brain in Wistar rats focal cerebral ischemia, regulate the plasticity of cerebral neuron.