摘要
目的研究一氧化氮(NO)对体外培养的骨肉瘤细胞株表面CD44表达的影响及意义。方法在体外培养骨肉瘤细胞株培养液中加入不同浓度的硝普钠(SNP)以产生外源性NO,用免疫组织化学的方法研究细胞表面CD44抗原成分的变化。结果肿瘤细胞表面CD44抗原在SNP存在的情况下表达明显下降。结论NO可以一定程度上抑制骨肉瘤细胞株CD44的表达,可能引起肿瘤细胞转移能力的改变。
Objective To study the influence of nitric oxide on the expression of CD44 of human osteosarcoma cell line （HOS）. Methods Different concentrations of sodium nitroprusside （SNP） were added into the culture solution of HOS in vitro. Through immunohistochemistry method （SP）, the expression of CD44 were observed. Results The expression of CD44 was depressed when the concentration of SNP was increased. Conclusion Nitric oxide can depress the expression of CD44 of HOS and maybe change the invasire or metastatic abilities of HOS.
出处
《中国康复理论与实践》
CSCD
2005年第7期552-552,F0003,共2页
Chinese Journal of Rehabilitation Theory and Practice
关键词
一氧化氮
骨肉瘤
硝普钠
CD44
nitric oxide
Osteosarcoma
sodium nitroprusside
CD44
