摘要
A Pt-nanooarticle/carbon nanotube modified graphite electrode immobilized with cholesterol oxidase/sol＇-gel layer was developed for monitoring cholesterol.Using this electrode,cholesterol concentration（4.0×10^-6 tp 1.0×10^-4mol/L）could be determined accurately in the presence of ascorbic or uric acid,and the response time was rapid （〈 20 s）. This biosensor has high sensitivity and selectivity.
A Pt-nanooarticle/carbon nanotube modified graphite electrode immobilized with cholesterol oxidase/sol＇-gel layer was developed for monitoring cholesterol.Using this electrode,cholesterol concentration（4.0×10^-6 tp 1.0×10^-4mol/L）could be determined accurately in the presence of ascorbic or uric acid,and the response time was rapid （〈 20 s）. This biosensor has high sensitivity and selectivity.
基金
The project is supported by the National Natural Science Foundation of China(29975024,202750-34)
Key Project of Science and Technology of Zhejiang Province(2003C21024).The authors acknowledge the Instrumental Analysis Center of Zhejiang University for special measurements.
