摘要
Macroporous cross-linking chitosan layer coated on silica gel （CTS-SiO2） was prepared by phase inversion and polyethylene glycol （PEG）molecular imprinting methods. Formation of macroporous surtace was investigated by scanning electron microscopy （SEM） and BET analysis.The prepared bead was activated by reacting with 1,2-ethylene digiycidyl ether for introducing epoxy groups, and trypsin could be efficiently immobilized on the bead as a biospecific ligand.The bead bearing trypsin was employed to purify trypsin inhibitor （TIs） from egg white as affinity adsorbent.
Macroporous cross-linking chitosan layer coated on silica gel （CTS-SiO2） was prepared by phase inversion and polyethylene glycol （PEG）molecular imprinting methods. Formation of macroporous surtace was investigated by scanning electron microscopy （SEM） and BET analysis.The prepared bead was activated by reacting with 1,2-ethylene digiycidyl ether for introducing epoxy groups, and trypsin could be efficiently immobilized on the bead as a biospecific ligand.The bead bearing trypsin was employed to purify trypsin inhibitor （TIs） from egg white as affinity adsorbent.
基金
The work is partially supported by the National Natural Science Foundation of China(20277031).
