波谱分析在沙棘果多糖H_n结构研究中的应用

Spectrum analysis of H_n, a polysaccharide extracted from the fruits of Hippophae rhamnoides polysaccharide

以波谱分析为主要手段研究沙棘果多糖Hn的结构,GC分析表明 Hn由Ara,Gal,Man和Glc组成,单糖物质的量比与利用GC分析Hn甲基化产 物的结果一致．13C-NMR分析表明Hn中存在α,β型糖苷键．利用GC-MS分 析进一步确定Hn主链结构由β(1→4)Glc,β(1→6)Man和β(1→4)Gal构成,在 3-O处有分支,支链由α(1→4)Glc,(1→6)Gal或末端Ara,Glc构成．

Spectrum analysis was the main tool to study the structure of the polysaccharide of Hippophae rhamnoides（H.）. GC analysis indicated that H. was consist of Ara,Gal, Man and Glc. The molar ratio of the monosaccharides attained by means of the analysis of methylated products is basically the same as attained by the GC analysis of GIA. ^13C-NMR revealed that Hn have the linkage of a and β configurations, From GC-MS,the main chain of H. is mainly composed of β（1→4）Glc,β（1→6）Man and β（1→4）Gal. The side chain was substituted at the 3-O.α（1→4）Glc and（1→6）Gal consist of the branch chain,the end of the residues of Hn is Ara and Glc.