还原型谷胱甘肽对新生鼠高氧肺纤维化早期干预的研究

The study of earlier peiod intervention of GSH on hyperoxic lung fibrosis in neonatal rats

目的研究还原型谷胱甘肽(GSH)对新生鼠高氧肺纤维化的影响,并观察转化生长因子-β1(transforminggrowthfactor-β1,TGF-β1)在高氧肺纤维化中的作用。方法将足月SD新生大鼠随机分为:I组空气对照组,Ⅱ组高氧组,Ⅲ组高氧+GSH组。观察及检测高氧暴露3、7、14、28d时各组肺组织病理改变、TGF-β1免疫组织化学染色。结果Ⅱ组3、7d时表现为明显的急性炎症改变,肺组织水肿、渗出、出血、肺泡间隔轻度增厚,14d时急性炎症减轻,而肺组织中TGF-β1表达呈强阳性;28d时出现明显的胶原沉积,形成纤维化;Ⅲ组与I组肺组织病理改变类似,Ⅲ组肺组织的TGF-β1表达呈弱阳性,无明显纤维化反应,两组无显著差异。结论TGF-β1在高氧性肺纤维化中可能起了重要作用,GSH早期干预高氧性肺纤维有一定作用,可以明显减轻肺纤维化的发生。

Objective To study effect of GSH hyperoxic lung injury in neonatal rats and to investigate the role of transforming growth factor-β1 （TGF-β1） in hyperoxic lung fibrosis. Methods The neonatal rats were randomly divided into 3 groups：group Ⅰ air control,group Ⅱ hyperoxia control,group Ⅲ hyperoxia ＋ GSH. At the end of the exposure, the lung histophatholoical changes were examined in all groups in 3d,7d,14d,28d. Results At 3d,7d of the exposure,there were obvious acute inflammation and wider alveolus alternation：edema, bleeding and strong express of TGF-β1 in group Ⅱ ;at 28d of exposure,there were obvious lung fibrosis. Lung pathologic changes in group Ⅲ were similar to those in group Ⅰ and there were no significant fibrosis regeneration. Conclusion TGF-β1 may have important role in hyperoxic-induce lung fiborsis injury. The earlier period intervention of GSH can reduce lung fibrosis regeneration and exceptional alveolus development.