高转移的人巨细胞肺癌PG细胞株血行转移的裸鼠模型的初步研究

The pilot study on metastasis model of highly metastasic human large cell lung cancer cell lines in nude mice

目的应用高转移的人巨细胞肺癌PG细胞株建立血行转移裸鼠模型,为探索肺癌转移的分子机制提供模型。方法用高转移的人巨细胞肺癌PG细胞株经左心室接种棵鼠,采用MRI观察转移灶形成时间、部位,12周后取转移灶进行体外细胞培养,流式细胞仪检测HLA-ABC蛋白的荧光强度。检测母代细胞、转移灶培养细胞下列指标:生长曲线、克隆形成率、细胞周期分析,并进行比较。结果接种6周时,在MRI下可见裸鼠有转移灶形成。10周时有30%裸鼠形成转移灶。MRI提示转移灶位于脑、胸椎、左肾上腺。母代细胞、转移灶培养细胞在生长曲线、克隆形成率、细胞周期无显著差异。结论高转移的人巨细胞肺癌PG细胞株血行转移的裸鼠模型的建立,为研究肺癌转移提供了新的实验模型。

Objective To establish metastasis model of highly metastasic human large cell lung cancer cell lines in nude mice so as to provide suitable model for the study of metastasis of lung cancer related molecular mechanism. Methods The highly metastasic human large cell lung cancer cell lines were inoculated into nude mice by left ventricle. Metastasic focus were observed by MRI in forming time and position. After 12 weeks, human tumor cells were recovered from metastasic focus, expanded in culture. After one to two weeks of culture, a pure population of human cancer cells was obtained, as confirmed by flow cytometry using a FITC-conjugated anti-human HLA-A, B, C antibody. In enatic cell and tumor cells of metastasic focus, the following parameters were studied ：in vitro growth curve,plate efficiency,an analysis of the cell cycle. Results Metastasic focus became detectable by MRI 6 weeks after inoculation,and appeared in 30% of inoculated animals after 10 weeks. Metastasic focus located brain,chest vertebra, left adrenal gland. Conclusion Metastasis model of highly metastasic human large cell lung cancer cell lines in nude mice has been established. It provides a new metastasis model in nude mice for the study of metastasis of lung cancer.