大鼠局灶性缺血预处理nestin表达的研究

The present of nestin after local brain ischemic preconditioning in rat

目的:观察局灶性缺血预处理(IPC)所诱导的脑缺血耐受(IT)现象及对中间丝蛋白(nestin)表达的影响。方法:采用线栓法局灶脑缺血模型35只Wistar大鼠随机分为PC+MCAO/MCAO/SS+MCAO三组,分别给予10min大脑中动脉缺血预处理(PC)、PC+2h大脑中动脉阻塞(MCAO)及假手术(SS)+2hMCAO,MCAO后24h处死,ABC免疫组化方法检测nestin表达。结果:nestin免疫反应阳性细胞数缺血预处理组(IPC+MCAO)明显高于单纯局灶缺血组(SS+MCAO),阳性细细胞呈棕褐色,清晰可辨,IPC+MCAO组nestin蛋白阳性细胞在缺血1d表达开始增多,3d达高峰,7d下降,MCAO组nestin蛋白阳性细胞表达时相一致,但明显降低。结论:10min大脑中动脉预缺血不会引起神经损害,但已足以诱导缺血耐受的产生;缺血耐受作用出现于PC后1d,缺血预处理可能通过增高nestin表达,对缺血引起的神经损伤起到保护作用。

Objective In order to investigate and compare the differences about nestin after focal ischemic preconditiomng（IPC） and ischemic in rat brain. Method the study using in Longa＇ rat model observed the localization of nestin in rat with anti-nestin polyclonal ABC immunohistochemica staining technique. Results it was shown that the positive nestin immunoreactivity was present higher in IPC＋MCAO group than MCAO group,and little present in SS＋SS group. Conclusion nestin shown high present after focal ischemic preconditioning and may play an important role protect against neurological impairment .