产嘌呤核苷的枯草杆菌prsA基因在大肠杆菌中的表达

Expression of a PrsA Gene from Purine Nucleoside Producing B.subtilis in E.coli

为了克隆产嘌呤核苷的枯草杆菌prsA基因。用PCR扩增的方法,从产肌苷的枯草杆菌BacillussubtilisJSIM-1019中克隆出一个长1kb长的DNA片段,经功能检测,证明正向插入片段与大肠杆菌的磷酸核糖焦磷酸营养缺陷特性(PRPP-)能够营养互补。含有该重组质粒的PRPP缺陷大肠杆菌JSIM-DH-27在基本培养基上的能够生长。

For cloning a prsA gene from purine nucleoside producing B. subtilis, one kb fragments of DNA was made to replicate from the chromosomal DNA of Bacillus subtilis JSIM- 1019 by the Polymerase Chain Reaction （PCR）. The functional examination demonstrated that the fragment was forward inserted into gene of the PRPP auxotroph mutant E. coli, and the E. coli JSIM- DH - 27 could grow on the minimal medium which did not contain PRPP.