赤藓糖醇发酵工艺研究

Study on Fermentation Process of Erythritol

赤藓糖醇的制备方法主要为微生物发酵法。为提高圆酵母(Torulasp.)B84512转化葡萄糖生产赤藓糖醇的发酵产量,采用控制发酵过程中葡萄糖起始浓度(30.0%)、中间流加葡萄糖浆的工艺使总葡萄糖糖浓度达到40%,发酵120h可产赤藓糖醇162.5gL,生产率为1.35gL·h,而不采取中间流加工艺,起始葡萄糖浓度为40.0%,发酵120h可产赤藓糖醇120gL,生产率为1.00gL·h。通过研究发现在赤藓糖醇发酵过程中采取中间流加葡萄糖工艺,能大大提高赤藓糖醇的发酵水平,提高幅度达30-35%,生产率也有相应提高。

Major manufacture method of erythritol was fermentation process by microorganism. Erythritol production was increased by control the initial concentration of glucose （30.0%） and use fed - batch glucose fermentation process . After 120 hrs , the yield of erythritol was increased to 162.5 g·l^-1 with a rate of 1.35 g·l^-1 h^-1 .Used the fed - batch glucose fermentation process , the concentration of erythritol production was increased 30% - 35% and the product rate as so.