摘要
对不同花色一串红组织培养快繁方法进行了研究,实验确定了一串红组织培养的最佳外植体为带腋芽的茎段,茎段的芽丛诱导的最佳培养基为12MS+NAA0.1mgL+6-BA2.0mgL+IBA0.5mgL,其分化率为71.4%,芽增殖率为140.6%;最佳生根培养基为14MS+NAA0.5mgL,生根率为91.6%,初步建立了快繁体系。
In this study 1 determined the best system of tissue culture fast propagations system,the result shows：The best explant in the culture was stem segment with bud,and the best medium of buds inducing was 1/2 MS＋NAA 0.1mg/L＋6-BA 2 mg/L＋IBA0.5mg/L,differentiation rate was 71.4%,buds multiplication rate was 140.62%.The best of rooting medium was 1/4MS＋NAA0.5mg/L,taking root rate was 91.6%.
出处
《生物技术》
CAS
CSCD
2005年第4期71-73,共3页
Biotechnology
关键词
一串红
外植体
组织培养
快繁体系
褐化
Salira splendens
explant
tissue culture
fast propagations system establisheb
browing
