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婴儿肠道细菌及乳杆菌种群多样性研究 被引量：1

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摘要 目的对婴儿肠道细菌及乳杆菌组成进行分析.方法以66例出生1～7 d的婴儿为调查对象,用分子生物学的手段,借助肠道细菌DNA指纹图谱和16S rDNA PCR扩增克隆分类(RFLP)技术.结果婴儿肠道菌群随出生天数增加而变化;乳杆菌特异性引物对PCR扩增结果表明,出生4 h后乳杆菌已在婴儿肠道内定植,且定植时间与分娩方式无关;克隆多样性研究则显示婴儿肠道乳杆菌优势菌群为植物乳杆菌(74%)、嗜酸乳杆菌(14%)和约氏乳杆菌(12%).结论婴儿肠道中的优势乳杆菌主要为植物乳杆菌.
作者 张和春 杨国 王劲松 杭晓敏 范小兵 王雁宇 朱志恒
机构地区 上海交大昂立股份有限公司生物医药研究所 上海交通大学生命科学与技术学院
出处 《中国微生态学杂志》 CAS CSCD 2005年第4期276-277,共2页 Chinese Journal of Microecology
关键词 婴儿 肠道细菌 乳杆菌 分子生物学
分类号 R378.992 [医药卫生—病原生物学]
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共引文献106

同被引文献11

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中国微生态学杂志
2005年 第4期

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