期刊文献+

IgA肾病患者核心1β3-半乳糖基转移酶基因编码区序列分析 被引量:1

The sequence analysis of core 1 β3-Galactosyltransferase gene coding region in IgA nephropathy
下载PDF
导出
摘要 目的:测定核心1β3-半乳糖基转移酶基因的DNA序列,试图在IgA肾病(IgAN)患者中发现该基因编码区DNA序列的异常。方法:应用双脱氧末端终止DNA测序技术,对核心1β3-半乳糖基转移酶基因外显子的PCR扩增产物进行直接测序,通过病例-对照研究方法,比较IgA肾病患者(50例)和正常对照(50例)之间核心1β3-半乳糖基转移酶基因组序列之间的差异。结果:两者的核心1β3-半乳糖基转移酶基因编码区序列无差异。结论:未发现IgA肾病患者有核心1β3-半乳糖基转移酶基因序列的异常,提示该基因编码区与IgA肾病患者IgA1的O-糖基化异常可能无关。 Objective:To sequence core 1β3-Galactosyltransferase gene in order to sought an abnormality in coding region sequence of the sequence in IgA nephropathy(IgAN). Methods:We used dideoxy-sequencing of the PCR-amplified exons of core 1β3-Galactosyltransferase gene to compare the sequences in IgA nephropathy (50 patients ) and controls (50 persons ).Results:The sequence of core 1 133-Galactosyltransferase gene coding region was identical in IgAN and controls. Conclusion.We found no abnormality in coding region sequence of core 1β3-galactosyltransferase gene, suggesting that this coding region probably not contribute to the decreased activity of core 1β3-galactosyltransferase.
出处 《军医进修学院学报》 CAS 北大核心 2005年第4期288-289,共2页 Academic Journal of Pla Postgraduate Medical School
基金 国家自然科学基金资助项目(30121005)
关键词 转移酶类 肾小球肾炎 IGA 散在重复序列 transferases glomerulonephiritis, IGA interspersed repetitive sequences
  • 相关文献

参考文献5

  • 1Allen AC, Topham PS, Harper SJ, et al. Leucocyte β1,3 galactosyltransferase activity in IgA nephropathy[J]. Nephrol Dial Transplant, 1997, 12: 701-706.
  • 2Tongzhong Ju, Kevin Brewer, Anil D Souza,et al. Cloning and Expression of Human core 1β1,3-Galactosyltransferase[J].J Bio Chem, 2002, 277(1):178-186.
  • 3Allen AC,Harper SJ,Feehally J.Galactosylation of N- and O-linked carbohydrate moeties of IgA1 and IgG in IgA nephropathy[J].Clin Exp Immunol, 1995, 100(3):470-474.
  • 4Jefferis R. The glycosylation of antibody molecules: functional significance[J]. Glycoconjugate J,1993,10: 358-361.
  • 5Morag R G, Jonathan B, Steven J H, et al. The nucleotide sequence of the IgA1 hinge region in IgA nephropathy[J]. Nephrol Dial Transplant, 1998, 13:1980-1983.

同被引文献2

引证文献1

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部