建立新生鼠缺氧缺血性脑病模型的一种简单可靠的方法

A Simple and Dependable Approach to Establish Hypoxic-Ischemic Encephalopathy Model in Neonatal Rat

目的探讨建立新生鼠缺氧缺血性脑病(HIE)动物模型快速可靠的方法。方法新生7 d SD乳鼠21只随机分为正常对照组、单纯缺氧组及缺氧缺血组。每组7只。每组又根据处死时间点随机分成3,6 h,1,3,7,14,21 d时7个小组。缺氧缺血组:结扎新生7 d SD大鼠左颈总动脉,置于8%浓度的低氧环境中2.5 h。单纯缺氧组缺氧2.5 h。采用HE染色及光镜技术观察3组SD大鼠脑组织结构改变。结果缺氧缺血组3 h出现轻度脑损伤,d1病变最严重,d3出现胶质细胞增生,142、1 d出现神经元大量丢失,皮层、纹状体、海马丘脑等部位形成胶质瘢痕,病灶中心仍可见大量固缩核和核碎片,7 d病灶中心仅见少量固缩核,周围增生的星形胶质细胞较3 d时增多。结论左颈总动脉结扎后置8%浓度低氧环境是简单、快速、可靠制备HIE模型的方法,且重复性好。

Objective To investigate a simple and dependable approach to establish the hypoxic-ischenic encephalopathy model in neonatal rat. Methods Twenty-one neonatal rats of 7 days old were randomly divided into control group, hypoxic group, and hypxicischemic group. Every group was randomly divided into 3 hours,6 hours, 1 day. 3 days, 7 days, 14 days,and 21 days group, according to the time of killing. Left common carotid artery of neonatal rats at age of 7 days in hypoxic-ischemic group were ligated. Then. the rats in hypoxic and hypoxic-ischemic group were put in a state of 8% oxygen for 2.5 hours. Brain tissues of rats in 3 groups were observed with HE staining under light microscope. Results In hypoxic-ischemic group, there was found mild brain damage after hypoxicischomic 3 hours, the brain lesion was most severe at 1 day, glial cell proliferation was found at 3 days, much neur were losed at 14,21 days, and colloid scar was formed in cortex, striatum and hippocampi. Conclusion The method thin left common carotid ornery of neonatal rats were ligated and then put in 8% oxygen for 2.5 hours is simple, rapid and dependable, which can be applied widely.